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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
16/02/2017 |
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Actualizado : |
24/06/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
PHAN, T.G; GIANNITTI, F.; ROSSOW,S.; MARTHALER,D.; KNUTSON, T.; LI, L.; DENG, X.; RESENDE, T.; VANNUCCI, F.; DELWART, E. |
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Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Detection of a novel circovirus PCV3 in pigs with cardiac and multi-systemic inflammation. |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
Virology Journal, 2016 v.13 n. 1. p. 184 |
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DOI : |
10.1186/s12985-016-0642-z |
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Idioma : |
Inglés |
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Notas : |
OPEN ACCESS |
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Contenido : |
Abstract.
Background: Porcine circovirus 2 causes different clinical syndromes resulting in a significant economic loss in the
pork industry. Three pigs with unexplained cardiac and multi-organ inflammation that tested negative for PCV2 and
other known porcine pathogens were further analyzed.
Methods: Histology was used to identify microscopic lesions in multiple tissues. Metagenomics was used to detect
viral sequences in tissue homogenates. In situ hybridization was used to detect viral RNA expression in cardiac tissue.
Results: In all three cases we characterized the genome of a new circovirus we called PCV3 with a replicase and capsid
proteins showing 55 and 35 % identities to the genetically-closest proteins from a bat-feces associated circovirus and
were even more distant to those of porcine circovirus 1 and 2. Common microscopic lesions included non-suppurative
myocarditis and/or cardiac arteriolitis. Viral mRNA was detected intralesionally in cardiac cells. Deep sequencing in
tissues also revealed the presence of porcine astrovirus 4 in all three animals as well as rotavirus A, porcine cytomegalovirus
and porcine hemagglutinating encephalomyelitis virus in individual cases.
Conclusion: The pathogenicity and molecular epidemiology of this new circovirus, alone or in the context of
co-infections, warrants further investigations. |
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Palabras claves : |
ARTERIOLITIS; ARTEROLITIS; CIRCOVIRUS; METAGENÓMICA; METAGENOMICS; MIOCARDITIS; PIG; VASCULITIS. |
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Thesagro : |
CERDO. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/6477/1/Virology-Journal-2016-13.p.184-Phan-et-al..pdf
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Marc : |
LEADER 02290naa a2200361 a 4500
001 1056664
005 2021-06-24
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1186/s12985-016-0642-z$2DOI
100 1 $aPHAN, T.G
245 $aDetection of a novel circovirus PCV3 in pigs with cardiac and multi-systemic inflammation.$h[electronic resource]
260 $c2016
500 $aOPEN ACCESS
520 $aAbstract. Background: Porcine circovirus 2 causes different clinical syndromes resulting in a significant economic loss in the pork industry. Three pigs with unexplained cardiac and multi-organ inflammation that tested negative for PCV2 and other known porcine pathogens were further analyzed. Methods: Histology was used to identify microscopic lesions in multiple tissues. Metagenomics was used to detect viral sequences in tissue homogenates. In situ hybridization was used to detect viral RNA expression in cardiac tissue. Results: In all three cases we characterized the genome of a new circovirus we called PCV3 with a replicase and capsid proteins showing 55 and 35 % identities to the genetically-closest proteins from a bat-feces associated circovirus and were even more distant to those of porcine circovirus 1 and 2. Common microscopic lesions included non-suppurative myocarditis and/or cardiac arteriolitis. Viral mRNA was detected intralesionally in cardiac cells. Deep sequencing in tissues also revealed the presence of porcine astrovirus 4 in all three animals as well as rotavirus A, porcine cytomegalovirus and porcine hemagglutinating encephalomyelitis virus in individual cases. Conclusion: The pathogenicity and molecular epidemiology of this new circovirus, alone or in the context of co-infections, warrants further investigations.
650 $aCERDO
653 $aARTERIOLITIS
653 $aARTEROLITIS
653 $aCIRCOVIRUS
653 $aMETAGENÓMICA
653 $aMETAGENOMICS
653 $aMIOCARDITIS
653 $aPIG
653 $aVASCULITIS
700 1 $aGIANNITTI, F.
700 1 $aROSSOW,S.
700 1 $aMARTHALER,D.
700 1 $aKNUTSON, T.
700 1 $aLI, L.
700 1 $aDENG, X.
700 1 $aRESENDE, T.
700 1 $aVANNUCCI, F.
700 1 $aDELWART, E.
773 $tVirology Journal, 2016$gv.13 n. 1. p. 184
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
29/07/2020 |
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Actualizado : |
05/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CASTELLS, M.; CAFFARENA, D.; CASAUX, M.L.; SCHILD, C.; MIÑO, S.; CASTELLS, F.; CASTELLS, D.; VICTORIA, M.; RIET-CORREA, F.; GIANNITTI, F.; PAREÑO, V.; COLINA, R. |
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Afiliación : |
MATÍAS CASTELLS BAUER, Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto 50000, Uruguay./INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./ Facultad de Veterinaria, Universidad de la República, Alberto Lasplaces 1620, Montevideo 11600, Uruguay.; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS SCHILD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SAMUEL MIÑO, Sección de Virus Gastroentéricos, Instituto de Virología, CICVyA, INTA Castelar, Buenos Aires 1686, Argentina.; FELIPE CASTELLS, Doctor en Veterinaria en Ejercicio Libre, Asociado al Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto 50000, Uruguay.; DANIEL CASTELLS, Centro de Investigación y Experimentación Dr. Alejandro Gallinal, Secretariado Uruguayo de la Lana, Ruta 7 km 140, Cerro Colorado, Florida 94000, Uruguay.; MATIAS VICTORIA, Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto 50000, Uruguay.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIVIANA PAREÑO, Sección de Virus Gastroentéricos, Instituto de Virología, CICVyA, INTA Castelar, Buenos Aires 1686, Argentina; RODNEY COLINA, Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto 50000, Uruguay. |
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Título : |
Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Pathogens (Basel, Switzerland), July 2020, Volume 9, Issue 7, Article number 570, Pages 1-17. OPEN ACCESS. DOI: https://doi.org/10.3390/pathogens9070570 |
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DOI : |
10.3390/pathogens9070570 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 7 April 2020 / Revised: 20 June 2020 / Accepted: 30 June 2020 / Published: 14 July 2020.
This research was funded by Instituto Nacional de Investigación Agropecuaria (INIA), grant number PL_015 N-15156 and N-23398, and by the Universidad de la República Program Polo de Desarrollo Universitario. The APC was funded by Universidad de la República. |
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Contenido : |
Abstract:
Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livestock industry. The aims of this study are to determine the frequency of RVA infections, and to analyze the genetic diversity of RVA strains in calves in Uruguay. A total of 833 samples from dairy and beef calves were analyzed through RT-qPCR and sequencing. RVA was detected in 57.0% of the samples. The frequency of detection was significantly higher in dairy (59.5%) than beef (28.4%) calves (p < 0.001), while it did not differ significantly among calves born in herds that were vaccinated (64.0%) or not vaccinated (66.7%) against NCD. The frequency of RVA detection and the viral load were significantly higher in samples from diarrheic (72.1%, 7.99 log10 genome copies/mL of feces) than non-diarrheic (59.9%, 7.35 log10 genome copies/mL of feces) calves (p < 0.005 and p = 0.007, respectively). The observed G-types (VP7) were G6 (77.6%), G10 (20.7%), and G24 (1.7%), while the P-types were P[5] (28.4%), P[11] (70.7%), and P[33] (0.9%). The G-type and P-type combinations were G6P[11] (40.4%), G6P[5] (38.6%), G10P[11] (19.3%), and the uncommon genotype G24P[33] (1.8%). VP6 and NSP1-5 genotyping were performed to better characterize some strains. The phylogenetic analyses suggested interspecies transmission, including transmission between animals and humans. MenosAbstract:
Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livestock industry. The aims of this study are to determine the frequency of RVA infections, and to analyze the genetic diversity of RVA strains in calves in Uruguay. A total of 833 samples from dairy and beef calves were analyzed through RT-qPCR and sequencing. RVA was detected in 57.0% of the samples. The frequency of detection was significantly higher in dairy (59.5%) than beef (28.4%) calves (p < 0.001), while it did not differ significantly among calves born in herds that were vaccinated (64.0%) or not vaccinated (66.7%) against NCD. The frequency of RVA detection and the viral load were significantly higher in samples from diarrheic (72.1%, 7.99 log10 genome copies/mL of feces) than non-diarrheic (59.9%, 7.35 log10 genome copies/mL of feces) calves (p < 0.005 and p = 0.007, respectively). The observed G-types (VP7) were G6 (77.6%), G10 (20.7%), and G24 (1.7%), while the P-types were P[5] (28.4%), P[11] (70.7%), and P[33] (0.9%). The G-type and P-type combinations were G6P[11] (40.4%), G6P[5] (38.6%), G10P[11] (19.3%), and the uncommon genotype G24P[33] (1.8%). VP6 and NSP1-5 genotyping were performed to better characterize some strains. The phylogenetic analyses suggested intersp... Presentar Todo |
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Palabras claves : |
BOVINE; DIARRHEA; GENOTYPES; INTERSPECIES TRANSMISSION; PLATAFORMA SALUD ANIMAL; ROTAVIRUS. |
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Thesagro : |
GANADO BOVINO. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16692/1/pathogens-09-00570.pdf
https://www.mdpi.com/2076-0817/9/7/570/pdf
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Marc : |
LEADER 03089naa a2200361 a 4500
001 1061250
005 2022-09-05
008 2020 bl uuuu u00u1 u #d
024 7 $a10.3390/pathogens9070570$2DOI
100 1 $aCASTELLS, M.
245 $aPhylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión.$h[electronic resource]
260 $c2020
500 $aArticle history: Received: 7 April 2020 / Revised: 20 June 2020 / Accepted: 30 June 2020 / Published: 14 July 2020. This research was funded by Instituto Nacional de Investigación Agropecuaria (INIA), grant number PL_015 N-15156 and N-23398, and by the Universidad de la República Program Polo de Desarrollo Universitario. The APC was funded by Universidad de la República.
520 $aAbstract: Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livestock industry. The aims of this study are to determine the frequency of RVA infections, and to analyze the genetic diversity of RVA strains in calves in Uruguay. A total of 833 samples from dairy and beef calves were analyzed through RT-qPCR and sequencing. RVA was detected in 57.0% of the samples. The frequency of detection was significantly higher in dairy (59.5%) than beef (28.4%) calves (p < 0.001), while it did not differ significantly among calves born in herds that were vaccinated (64.0%) or not vaccinated (66.7%) against NCD. The frequency of RVA detection and the viral load were significantly higher in samples from diarrheic (72.1%, 7.99 log10 genome copies/mL of feces) than non-diarrheic (59.9%, 7.35 log10 genome copies/mL of feces) calves (p < 0.005 and p = 0.007, respectively). The observed G-types (VP7) were G6 (77.6%), G10 (20.7%), and G24 (1.7%), while the P-types were P[5] (28.4%), P[11] (70.7%), and P[33] (0.9%). The G-type and P-type combinations were G6P[11] (40.4%), G6P[5] (38.6%), G10P[11] (19.3%), and the uncommon genotype G24P[33] (1.8%). VP6 and NSP1-5 genotyping were performed to better characterize some strains. The phylogenetic analyses suggested interspecies transmission, including transmission between animals and humans.
650 $aGANADO BOVINO
653 $aBOVINE
653 $aDIARRHEA
653 $aGENOTYPES
653 $aINTERSPECIES TRANSMISSION
653 $aPLATAFORMA SALUD ANIMAL
653 $aROTAVIRUS
700 1 $aCAFFARENA, D.
700 1 $aCASAUX, M.L.
700 1 $aSCHILD, C.
700 1 $aMIÑO, S.
700 1 $aCASTELLS, F.
700 1 $aCASTELLS, D.
700 1 $aVICTORIA, M.
700 1 $aRIET-CORREA, F.
700 1 $aGIANNITTI, F.
700 1 $aPAREÑO, V.
700 1 $aCOLINA, R.
773 $tPathogens (Basel, Switzerland), July 2020, Volume 9, Issue 7, Article number 570, Pages 1-17. OPEN ACCESS. DOI: https://doi.org/10.3390/pathogens9070570
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